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custom-made multiplex solid phase microarray platform  (Thermo Fisher)


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    Thermo Fisher custom-made multiplex solid phase microarray platform
    Custom Made Multiplex Solid Phase Microarray Platform, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+microarray/custom+made+multiplex+solid+phase+microarray+platform/pm40116570-69-14-19
    Average 90 stars, based on 1 article reviews
    custom-made multiplex solid phase microarray platform - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: COPD GWAS variant at 19q13.2 in relation with DNA methylation and gene expression
    Article Snippet: Illumina Human1M-Duo BeadChip arrays were used for genotyping, and a custom Affymetrix microarray (GPL10379) for gene expression profiling.

    Article Title: A Genome-Wide Analysis of Small Regulatory RNAs in the Human Pathogen Group A Streptococcus
    Article Snippet: To facilitate identification of candidate sRNAs transcribed by the serotype M1 GAS strain MGAS2221 we designed a custom Affymetrix microarray.

    Article Title: Regulatory Patterns of a Large Family of Defensin-Like Genes Expressed in Nodules of Medicago truncatula
    Article Snippet: Ten micrograms of biotin-labeled cRNA, fragmented as suggested by Affymetrix, was hybridized to a custom Affymetrix microarray, the AtMtDEFL array .

    Article Title: Method and cells for the production of viral vaccines
    Article Snippet: Briefly, DNA was isolated, shRNA sequences were PCR-amplified and hybridized to a custom Affymetrix microarray to quantify hairpin abundance.

    Article Title: A Genome-Wide Analysis of Small Regulatory RNAs in the Human Pathogen Group A Streptococcus
    Article Snippet: Genome-wide analysis of GAS gene expression was achieved through use of a custom Affymetrix microarray that contained 16 antisense oligonucleotide probe pairs (PM + MM) for each gene in the MGAS2221 genome.

    Article Title: Indole signalling and (micro)algal auxins decrease the virulence of Vibrio campbellii, a major pathogen of aquatic organisms.
    Article Snippet: Author affiliations: 1 Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Gent, Belgium 2 Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington DC, USA 3 Mathematics Department, Whittier College, Whittier, CA, USA 4 Center for Microbial Ecology and Technology (cmet), Ghent University, Gent, Belgium a Current address: Defense Threat Reduction Agency, Chemical and Biological Technologies, Fort Belvoir, VA, USA

    Selection:

    Article Title: Altered extracellular matrix and mechanotransduction gene expression in rat bone tissue following long-term estrogen deficiency
    Article Snippet: A customized microarray (Applied Biosciences) was designed to study (1) bone anabolism and extracellular matrix genes (Spp1, OPG, COL1A1, COL1A2, OCN, LRP5, Alpl, Tgfβ1, Lox, Ager, DMP1, PHEX, Klf10, Fn1), (2) bone catabolism and extracellular matrix degradation genes (RANKL, RANK, Nfatc-1, Mmp9, Mmp13, Sost, CtsK, Wnt10B, CCR2, IL1B, TP53, Casp3, Lamp1, ATG7, Rptor), and (3) mechanotransduction and mechanosensation genes (Vcl, Itga5, Itgav, Itgb1, Cx43, Esr1, Axin2, IFT88, TRPV4, Adcy6, Ryr, Pkd1). .. The selection and categorization of genes were carried out based on specific research objectives and the nature of the custom microarray (Applied Biosciences) used in the study. ..

    Microarray:

    Article Title: Altered extracellular matrix and mechanotransduction gene expression in rat bone tissue following long-term estrogen deficiency
    Article Snippet: A customized microarray (Applied Biosciences) was designed to study (1) bone anabolism and extracellular matrix genes (Spp1, OPG, COL1A1, COL1A2, OCN, LRP5, Alpl, Tgfβ1, Lox, Ager, DMP1, PHEX, Klf10, Fn1), (2) bone catabolism and extracellular matrix degradation genes (RANKL, RANK, Nfatc-1, Mmp9, Mmp13, Sost, CtsK, Wnt10B, CCR2, IL1B, TP53, Casp3, Lamp1, ATG7, Rptor), and (3) mechanotransduction and mechanosensation genes (Vcl, Itga5, Itgav, Itgb1, Cx43, Esr1, Axin2, IFT88, TRPV4, Adcy6, Ryr, Pkd1). .. The selection and categorization of genes were carried out based on specific research objectives and the nature of the custom microarray (Applied Biosciences) used in the study. ..

    Article Title: Transcriptional response of Mexican axolotls to Ambystoma tigrinum virus (ATV) infection
    Article Snippet: .. To further investigate the axolotl immune response to ATV, we used an Affymetrix custom microarray to identify genes that were significantly, differentially expressed in the spleen. ..



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    Image Search Results


    Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.

    Journal: Journal of Clinical Microbiology

    Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

    doi: 10.1128/jcm.01260-25

    Figure Lengend Snippet: Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.

    Article Snippet: Translated proteins and IVTT controls were printed onto custom nitrocellulose-coated microarray slides (Grace Bio-Labs, Bend, OR, USA) using a GeneMachines OmniGrid Accent Microarray Printer.

    Techniques: Microarray, Infection, Comparison

    Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.

    Journal: Journal of Clinical Microbiology

    Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

    doi: 10.1128/jcm.01260-25

    Figure Lengend Snippet: Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.

    Article Snippet: Translated proteins and IVTT controls were printed onto custom nitrocellulose-coated microarray slides (Grace Bio-Labs, Bend, OR, USA) using a GeneMachines OmniGrid Accent Microarray Printer.

    Techniques: Microarray, Infection, Comparison

    Dot-plot between anti-CRP antibodies and epitope positivity. The graph displays anti-CRP antibodies versus the number of positive epitopes obtained with microarray-based linear epitope mapping in patients with systemic lupus erythematosus ( n =42). (CRP, C-reactive protein).

    Journal: Frontiers in Immunology

    Article Title: Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation

    doi: 10.3389/fimmu.2025.1578372

    Figure Lengend Snippet: Dot-plot between anti-CRP antibodies and epitope positivity. The graph displays anti-CRP antibodies versus the number of positive epitopes obtained with microarray-based linear epitope mapping in patients with systemic lupus erythematosus ( n =42). (CRP, C-reactive protein).

    Article Snippet: The full sequence of the CRP monomer was printed in 15 a.a sequences with 14 a.a overlap using PEPperCHIP Custom Peptide Microarray (PEPperPRINT ® GmbH, Heidelberg, Germany).

    Techniques: Microarray

    Heatmap representation of autoreactivity against CRP obtained with microarray-based linear epitope mapping of the full CRP monomer. (A, B) Individual signal intensity of IgG autoantibody reactivity against full-length CRP for subjects with SLE ( n =42) and HBD ( n =11). (C–E) Mean signal intensity of IgG autoantibody reactivity against motifs of full-length CRP comparing anti-CRP negative (anti-CRP–; n =16) vs. anti-CRP positive (anti-CRP+; n =26) patients with SLE, patients with ( n =6) and without ( n =36) active disease, and no damage ( n =20) vs. irreversible organ damage (any organ system; n =22). Each column represents one subject (A, B) or the mean value of a group (C–E) . Each row represents a 15 amino acid long sequence covering the full length of the protein with 14 amino acids overlap and 7 amino acid GS repeats elongated before and after the protein sequence. (CRP, C-reactive protein; HBD, healthy blood donors; SDI, Systemic Lupus International Collaborating Clinics/American College of Rheumatology damage index; SLE, systemic lupus erythematosus; SLEDAI-2K, SLE Disease Activity Index 2000).

    Journal: Frontiers in Immunology

    Article Title: Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation

    doi: 10.3389/fimmu.2025.1578372

    Figure Lengend Snippet: Heatmap representation of autoreactivity against CRP obtained with microarray-based linear epitope mapping of the full CRP monomer. (A, B) Individual signal intensity of IgG autoantibody reactivity against full-length CRP for subjects with SLE ( n =42) and HBD ( n =11). (C–E) Mean signal intensity of IgG autoantibody reactivity against motifs of full-length CRP comparing anti-CRP negative (anti-CRP–; n =16) vs. anti-CRP positive (anti-CRP+; n =26) patients with SLE, patients with ( n =6) and without ( n =36) active disease, and no damage ( n =20) vs. irreversible organ damage (any organ system; n =22). Each column represents one subject (A, B) or the mean value of a group (C–E) . Each row represents a 15 amino acid long sequence covering the full length of the protein with 14 amino acids overlap and 7 amino acid GS repeats elongated before and after the protein sequence. (CRP, C-reactive protein; HBD, healthy blood donors; SDI, Systemic Lupus International Collaborating Clinics/American College of Rheumatology damage index; SLE, systemic lupus erythematosus; SLEDAI-2K, SLE Disease Activity Index 2000).

    Article Snippet: The full sequence of the CRP monomer was printed in 15 a.a sequences with 14 a.a overlap using PEPperCHIP Custom Peptide Microarray (PEPperPRINT ® GmbH, Heidelberg, Germany).

    Techniques: Microarray, Sequencing, Activity Assay